Detection of IL-10 gene polymorphisms in patients with ulcerative colitis in Northern Iran

Background: Ulcerative colitis is a multifactorial disease in which the environmental and genetics factor are being involved together. Interleukins are a group of cytokine which are produced through T cells, monocytes, macrophages, and endothelial cells. Interleukin-10 is an important multitasking cytokine that has a key role in inflammatory responses. The promoter of interlekin-10 is highly polymorphic and many of its polymorphisms were identified. The purpose of this study was to investigate and detect the A1082G and T819C polymorphisms of interleukin-10 promoter in ulcerative colitis patients. Materials and methods: In this case- control study, DNA from 150 individuals with ulcerative colitis and 150 controls were determined by PCR technique. Data were analyzed by Chi-square test using SPSS software. Also, odds ratios and 95% confidence interval were calculated. Results: The results showed that distribution of GG and CC genotypes and frequency of G and C alleles (related to A1082C and T819C polymorphisms, respectively) was significantly different between patient and control groups. Conclusion: According to the results, both polymorphisms of IL-10 gene promoter (A1082G and T819C), including G allel and C allel, respectively, are likely to be considered as risk factors for ulcerative colitis. Moreover, both genotype of GG and CC can increase the incidence of ulcerative colitis. Keywords: Ulcerative colitis, Polymorphism, IL-10 (A1082G), IL-10 (T819C).